COSMOSIL πNAP HPLC Columns

COSMOSIL πNAP is a reversed phase HPLC column with naphtylethyl group bonded silica packing material. The naphthylethyl group is composed of two fused aromatic rings and forms strong π-π interactions with unsaturated compounds. This column offers improved separation of compounds such as positional isomers that are difficult to analyze with alkyl group bonded materials.

 Main Characteristics

• Enhanced π- π interactions
• Improved selectivity for structural isomers
• Naphthalene bonded stationary phase

Comparison of π-π Interactions

COSMOSIL πNAP shows stronger π-π interactions than phenyl columns.

Applications

Tolunitrile

 

Condition
Column Size	4.6 mm I.D. x 150 mm
Flow Rate	1.0ml/min
Temperature	30°C
Detection	UV 254 nm
Sample	o-Tolunitrile 2.0 µg m-Tolunitrile 2.0 µg p-Tolunitrile 1.0 µg

 

Adrenal Cortical Hormone

 

Condition
Column Size	4.6 mm I.D. x 150 mm
Flow Rate	1.0ml/min
Temperature	30°C
Detection	UV 254 nm
Sample	Prednisolone 0.33 µg Hydrocortisone 0.33 µg Cortisone 0.33 µg

 

Berberine

 

Condition
Column Size	4.6 mm I.D. x 150 mm
Mobile Phase	Methanol : 20 mmol/l Phosphate Buffer (pH2.5) =                     5C18-MS-II 40 : 60                     5PE-MS  60 : 40 πNAP  80 : 20
Flow Rate	1.0ml/min
Temperature	30°C
Detection	UV 254 nm
Sample	Palmatine chloride 0.2 µg Berberine Hydrochoride 0.2 µg

 

Isoflavone

 

Condition
Column Size	4.6 mm I.D. x 150 mm	Flow rate	1.0ml/min
Mobile Phase	A Acetonitrile / 20mmol/l Phosphate Buffer (pH 2.5)=10/90       B Acetonitrile / 20mmol/l Phosphate Buffer (pH 2.5)=50/50       B Conc. 0 → 100% 20min Linear Gradient	Temperature	30°C
		Detection	UV 260 nm
		Sample	Daidzin 0.05 µg Glycitin 0.05 µg Genistin 0.05 µg Daidzein 0.05 µg Glycitein 0.075 µg Genistein 0.025 µg

 

Ordering Information

Particle Size	Description	Column Dimensions	Part Number
5um	COSMOSIL πNAP Packed Column	1.0mmI.D. x 150mm	08076-61
5um	COSMOSIL πNAP Packed Column	1.0mmI.D. x 250mm	08077-51
5um	COSMOSIL πNAP Packed Column	2.0mmI.D. x 30mm	08566-41
5um	COSMOSIL πNAP Packed Column	2.0mmI.D. x 50mm	08567-31
5um	COSMOSIL πNAP Packed Column	2.0mmI.D. x 100mm	08299-51
5um	COSMOSIL πNAP Packed Column	2.0mmI.D. x 150mm	08078-41
5um	COSMOSIL πNAP Packed Column	2.0mmI.D. x 250mm	08079-31
5um	COSMOSIL πNAP Packed Column	3.0mmI.D. x 150mm	08080-91
5um	COSMOSIL πNAP Packed Column	3.0mmI.D. x 250mm	08081-81
5um	COSMOSIL πNAP Packed Column	4.6mmI.D. x 50mm	08083-61
5um	COSMOSIL πNAP Packed Column	4.6mmI.D. x 100mm	08084-51
5um	COSMOSIL πNAP Packed Column	4.6mmI.D. x 150mm	08085-41
5um	COSMOSIL πNAP Packed Column	4.6mmI.D. x 250mm	08086-31
5um	COSMOSIL πNAP Packed Column	10.0mmI.D. x 150mm	08088-11
5um	COSMOSIL πNAP Packed Column	10.0mmI.D. x 250mm	08089-01
5um	COSMOSIL πNAP Packed Column	20.0mmI.D. x 150mm	08092-41
5um	COSMOSIL πNAP Packed Column	20.0mmI.D. x 250mm	08093-31
5um	COSMOSIL πNAP Packed Column	28.0mmI.D. x 250mm	08095-11
2.5um	COSMOSIL 2.5πNAP Packed Column	2.0mmI.D. x 50mm	06062-91
2.5um	COSMOSIL 2.5πNAP Packed Column	2.0mmI.D. x 70mm	06051-31
2.5um	COSMOSIL 2.5πNAP Packed Column	2.0mmI.D. x 100mm	06052-21
2.5um	COSMOSIL 2.5πNAP Packed Column	3.0mmI.D. x 50mm	06054-01
2.5um	COSMOSIL 2.5πNAP Packed Column	3.0mmI.D. x 70mm	06055-91
2.5um	COSMOSIL 2.5πNAP Packed Column	3.0mmI.D. x 100mm	06057-71
Guard Columns
5um	COSMOSIL πNAP Guard Column	4.6mmI.D. x 10mm	08082-71
5um	COSMOSIL πNAP Guard Column	10.0mmI.D. x 20mm	08087-21
5um	COSMOSIL πNAP Guard Column	20.0mmI.D. x 20mm	08090-61
5um	COSMOSIL πNAP Guard Column	20.0mmI.D. x 50mm	08091-51
5um	COSMOSIL πNAP Guard Column	28.0mmI.D. x 50mm	08094-21