Cosmosil COSMOSIL piNAP COSMOSIL πNAP HPLC Columns.. Product #: VEL00490

COSMOSIL piNAP

COSMOSIL piNAP

Brand: Cosmosil
Product Code: VEL00490

COSMOSIL πNAP HPLC Columns

COSMOSIL piNAP StructureCOSMOSIL πNAP is a reversed phase HPLC column with naphtylethyl group bonded silica packing material. The naphthylethyl group is composed of two fused aromatic rings and forms strong π-π interactions with unsaturated compounds. This column offers improved separation of compounds such as positional isomers that are difficult to analyze with alkyl group bonded materials.

 Main Characteristics

  • Enhanced π- π interactions
  • Improved selectivity for structural isomers
  • Naphthalene bonded stationary phase

 

 

COSMOSIL πNAP HPLC Columns
 Base Material Silica Gel
 Particle Sizes Available 2.5, 5um
 Pore Size 120Å
 Surface Area 330m2/g
 pH Range 2.0 - 8.0
 USP Code -
 Carbon Load 11%

 

Comparison of π-π Interactions

COSMOSIL πNAP shows stronger π-π interactions than phenyl columns.

 COSMOSIL piNAP 1

Applications

Tolunitrile

 COSMOSIL piNAP 2

Condition
Column Size 4.6 mm I.D. x 150 mm COSMOSIL piNAP 3
Flow Rate 1.0ml/min
Temperature 30°C
Detection UV 254 nm
Sample
  • o-Tolunitrile 2.0 µg
  • m-Tolunitrile 2.0 µg
  • p-Tolunitrile 1.0 µg
 
Adrenal Cortical Hormone

 COSMOSIL piNAP 4

Condition
Column Size 4.6 mm I.D. x 150 mm COSMOSIL piNAP 5
Flow Rate 1.0ml/min
Temperature 30°C
Detection UV 254 nm
Sample
  1. Prednisolone 0.33 µg
  2. Hydrocortisone 0.33 µg
  3. Cortisone 0.33 µg
 
Berberine

 COSMOSIL piNAP 6

Condition
Column Size 4.6 mm I.D. x 150 mm COSMOSIL piNAP 7
Mobile Phase

Methanol : 20 mmol/l Phosphate Buffer (pH2.5) =                    

5C18-MS-II 40 : 60                    

5PE-MS  60 : 40

πNAP  80 : 20

Flow Rate 1.0ml/min
Temperature 30°C
Detection UV 254 nm
Sample
  1. Palmatine chloride 0.2 µg
  2. Berberine Hydrochoride 0.2 µg
 
Isoflavone

 COSMOSIL piNAP 8

Condition
Column Size 4.6 mm I.D. x 150 mm Flow rate 1.0ml/min
Mobile Phase A Acetonitrile / 20mmol/l Phosphate Buffer (pH 2.5)=10/90       B Acetonitrile / 20mmol/l Phosphate Buffer (pH 2.5)=50/50       B Conc. 0 → 100% 20min Linear Gradient Temperature 30°C
Detection UV 260 nm
Sample
  1. Daidzin 0.05 µg
  2. Glycitin 0.05 µg
  3. Genistin 0.05 µg
  4. Daidzein 0.05 µg
  5. Glycitein 0.075 µg
  6. Genistein 0.025 µg
 

Ordering Information

Particle Size Description Column Dimensions Part Number
5um COSMOSIL πNAP Packed Column 1.0mmI.D. x 150mm 08076-61
5um COSMOSIL πNAP Packed Column 1.0mmI.D. x 250mm 08077-51
5um COSMOSIL πNAP Packed Column 2.0mmI.D. x 30mm 08566-41
5um COSMOSIL πNAP Packed Column 2.0mmI.D. x 50mm 08567-31
5um COSMOSIL πNAP Packed Column 2.0mmI.D. x 100mm 08299-51
5um COSMOSIL πNAP Packed Column 2.0mmI.D. x 150mm 08078-41
5um COSMOSIL πNAP Packed Column 2.0mmI.D. x 250mm 08079-31
5um COSMOSIL πNAP Packed Column 3.0mmI.D. x 150mm 08080-91
5um COSMOSIL πNAP Packed Column 3.0mmI.D. x 250mm 08081-81
5um COSMOSIL πNAP Packed Column 4.6mmI.D. x 50mm 08083-61
5um COSMOSIL πNAP Packed Column 4.6mmI.D. x 100mm 08084-51
5um COSMOSIL πNAP Packed Column 4.6mmI.D. x 150mm 08085-41
5um COSMOSIL πNAP Packed Column 4.6mmI.D. x 250mm 08086-31
5um COSMOSIL πNAP Packed Column 10.0mmI.D. x 150mm 08088-11
5um COSMOSIL πNAP Packed Column 10.0mmI.D. x 250mm 08089-01
5um COSMOSIL πNAP Packed Column 20.0mmI.D. x 150mm 08092-41
5um COSMOSIL πNAP Packed Column 20.0mmI.D. x 250mm 08093-31
5um COSMOSIL πNAP Packed Column 28.0mmI.D. x 250mm 08095-11
2.5um COSMOSIL 2.5πNAP Packed Column 2.0mmI.D. x 50mm 06062-91
2.5um COSMOSIL 2.5πNAP Packed Column 2.0mmI.D. x 70mm 06051-31
2.5um COSMOSIL 2.5πNAP Packed Column 2.0mmI.D. x 100mm 06052-21
2.5um COSMOSIL 2.5πNAP Packed Column 3.0mmI.D. x 50mm 06054-01
2.5um COSMOSIL 2.5πNAP Packed Column 3.0mmI.D. x 70mm 06055-91
2.5um COSMOSIL 2.5πNAP Packed Column 3.0mmI.D. x 100mm 06057-71
Guard Columns
5um COSMOSIL πNAP Guard Column 4.6mmI.D. x 10mm 08082-71
5um COSMOSIL πNAP Guard Column 10.0mmI.D. x 20mm 08087-21
5um COSMOSIL πNAP Guard Column 20.0mmI.D. x 20mm 08090-61
5um COSMOSIL πNAP Guard Column 20.0mmI.D. x 50mm 08091-51
5um COSMOSIL πNAP Guard Column 28.0mmI.D. x 50mm 08094-21