Cosmosil COSMOSIL C18-MS-II COSMOSIL C18-MS-II HPLC Columns.. Product #: VEL00473

COSMOSIL C18-MS-II

COSMOSIL C18-MS-II

Brand: Cosmosil
Product Code: VEL00473

COSMOSIL C18-MS-II HPLC Columns

The COSMOSIL C18-MS-II is a well-balanced column with better basic performance such as sharper peaks of basic compounds and chelating compounds, and high theoretical plate number. It is the most popular HPLC column because we produce very consistent products and minimize variation from lot to lot. Furthermore we provide abundant Applications with the column, so it will help you to choose an analysis condition of your sample.

 Main Characteristics

  • High Durability and increased Peak Shape for Basic Compounds
  • Extended pH Range
  • Perfect for low molecular weight Organic Compounds
COSMOSIL C18-MS-II
 Base Material Silica Gel
 Particle Sizes Available 2.5, 3, 5, 15um
 Pore Size 120Å
 Surface Area 330m2/g
 pH Range 2.0 - 10.0
 USP Code L1
 Carbon Load 16%

 

Analysis of Basic Compounds

The COSMOSIL C18-MS-II column, taking advantage of a new end-capping treatment, can replace the original COSMOSIL C18(ODS) column. A new end-capping treatment with polar groups for “shield effect” has significantly improved peak shape for basic compounds.

PROCAINAMIDE

COSMOSIL C18-MS-II 1

Condition
Column Size 4.6mm I.D. x 150 mm
Mobile Phase Methanol : 20 mmol/l                       Phosphate Buffer (pH7) = 20 : 80
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm
Sample
  1. Procainamide 0.38 µg
  2. N-Acetylprocainamide0.25 µg
  3. Benzyl Alcohol 5.63 µg
 
BENZYLAMINE

COSMOSIL C18-MS-II 2

Condition
Column Size 4.6 mm I.D. x 150 mm
Mobile Phase Methanol : 20 mmol/l                       Phosphate Buffer (pH7) = 20 : 80
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm
Sample
  1. Uracil
  2. Benzylamine
  3. N-Methylbenzylamine
  4. N,N-Dimethylbenzylamine
  5. Benzyl Alcohol
 
PYRIDINE

COSMOSIL C18-MS-II 3

Condition
Column Size 4.6 mm I.D. x 150 mm
Mobile Phase Acetonitrile : Water = 30 : 70
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm
Sample
  1. Pyridine 0.4 µg
  2. Phenol 1.7 µg

 

Stability under Alkaline Conditions

The durability under extremely high pH is greatly improved in the new COSMOSIL C18-MS-II compared with old COSMOSIL C18-MS. Although it is possible to use COSMOSIL under alkaline condition for a limited time, some deterioration may occur after long exposure. We recommend pH7.5 and below for long-term usage. Between the measurements, methanol : 20 mM/l tris buffer (pH10.0) = 50 : 50 mobile phase was run at 1.0 ml/min flow rate, no significant deterioration could be observed.

COSMOSIL C18-MS-II 6 COSMOSIL C18-MS-II 7
Retention factor for Naphthalene Theoretical plate numbers for Pyridine
Condition
Column Size 4. 6 mm I.D. x 150 mm
Mobile Phase Methanol : Water =70 : 30
Flow Rate 1.0 ml /min
Temperature 30 °C
Detection UV 254 nm
Sample Naphtalene
Condition
Column Size 4. 6 mm I.D. x 150 mm
Mobile Phase Acetonitrile : Water =30 : 70
Flow Rate 1.0 ml /min
Temperature 30 °C
Detection UV 254 nm
Sample Pyridine

 

Particle Size Description Column Dimensions Part Number
2.5um COSMOSIL 2.5C18-MS-II Column 2.0mmI.D. x 100mm 08996-11
2.5um COSMOSIL 2.5C18-MS-II Column 2.0mmI.D. x 50mm 08994-31
2.5um COSMOSIL 2.5C18-MS-II Column 2.0mmI.D. x 70mm 08995-21
2.5um COSMOSIL 2.5C18-MS-II Column 3.0mmI.D. x 100mm 08999-81
2.5um COSMOSIL 2.5C18-MS-II Column 3.0mmI.D. x 50mm 08997-01
2.5um COSMOSIL 2.5C18-MS-II Column 3.0mmI.D. x 70mm 08998-91
3um COSMOSIL 3C18-MS-II Column 2.0mmI.D. x 50mm 05514-01
3um COSMOSIL 3C18-MS-II Column 4.6mmI.D. x 100mm 38067-51
3um COSMOSIL 3C18-MS-II Column 4.6mmI.D. x 10mm 38065-71
3um COSMOSIL 3C18-MS-II Column 4.6mmI.D. x 50mm 38066-61
5um COSMOSIL 5C18-MS-II Column 1.0mmI.D. x 150mm 02896-01
5um COSMOSIL 5C18-MS-II Column 1.0mmI.D. x 50mm 02824-31
5um COSMOSIL 5C18-MS-II Column 10.0mmI.D. x 150mm 34355-91
5um COSMOSIL 5C18-MS-II Column 10.0mmI.D. x 250mm 38023-11
5um COSMOSIL 5C18-MS-II Column 10.0mmI.D. x 50mm 05789-21
5um COSMOSIL 5C18-MS-II Column 2.0mmI.D. x 100mm 05597-31
5um COSMOSIL 5C18-MS-II Column 2.0mmI.D. x 150mm 38025-91
5um COSMOSIL 5C18-MS-II Column 2.0mmI.D. x 250mm 05761-61
5um COSMOSIL 5C18-MS-II Column 2.0mmI.D. x 30mm 05876-71
5um COSMOSIL 5C18-MS-II Column 2.0mmI.D. x 50mm 04355-21
5um COSMOSIL 5C18-MS-II Column