COSMOSIL C18-EB HPLC Columns
Conventionally end-capped C18 columns still have many residual silanol groups on the silica surface of stationary phase that can form ionic bonds with basic compounds. The resulting peak tailing makes accurate quantification of analytes difficult, especially in trace analyses. COSMOSIL 3C18-EB with a better end-capping treatment offers improved peak shape and separation particularly for basic compounds.
Main Characteristics
- Improved separation for acidic or metal coordination compounds
- Simple mobile phase conditions
- Excellent for Basic Compounds
COSMOSIL C18-EB |
Base Material |
Silica Gel |
Particle Sizes Available |
3um |
Pore Size |
120A |
Surface Area |
300m2/g |
pH Range |
2-10 |
USP Code |
L1 |
Carbon Load |
14.5% |
Comparison (Basic compounds)
COSMOSIL C18-EB | Competitor A C18, 3 µm | Competitor B C18, 3 µm | Competitor C C18, 3 µm |
 |
 |
 |
 |
Conditions |
Column Size |
4.6 mm I.D. x 75 mm |
Mobile Phase |
Acetonitrile/ 20mmol/l Phosphate Buffer(pH7.0) = 60/40 |
Flow Rate |
1.0 ml/min |
Temperature |
40°C |
Detection |
UV 254 nm |
Sample |
1. Amitriptyline 2. Propylbenzene (I.S.) |
0.2mg/ml 2.0mg/ml |
 |
|
Inj. Vol |
2.0 µl |
Analysis of Acidic or Metal Coordination Compounds
Acidic or metal coordination compounds can also form bonds with residual silanol groups and metal impurities causing peak tailing. COSMOSIL 3C18-EB with a better end-capping treatment and high purity silica gel offers improved peak shape and separation for acidic or metal coordination compounds.
Acidic Compounds
COSMOSIL C18-EB |
 |
Conditions |
Column Size |
4.6 mm I.D. x 75 mm |
Mobile Phase |
Acetonitrile/ 20mmol/l Phosphate Buffer(pH2.5) = 30/70 |
Flow Rate |
1.0 ml/min |
Temperature |
40°C |
Detection |
UV254 nm |
Sample |
1. Salicylic Acid 2. Ethyl p-Hydroxybenzoate (I.S.) |
1.0 mg/ml 0.05 mg/ml |
 |
|
Metal Coordination Compounds
COSMOSIL C18-EB |
 |
Conditions |
Column Size |
4.6 mm I.D. x 75 mm |
Mobile Phase |
Acetonitrile/ 20mmol/l Phosphoric Acid = 10/90 |
Flow Rate |
1.0 ml/min |
Temperature |
40°C |
Detection |
UV 254 nm |
Sample |
1. Oxine-copper 2. Caffeine (I.S.) |
0.03 mg/ml 0.3 mg/ml |
 |
|
Inj. Vol |
2.0 µl |
Analysis under Ammonium Acetate Buffer
COSMOSIL C18-EB produces sharp peaks using ammonium acetate buffer in LC-MS.
Acetonitrile/ 20mmol/l Phosphate buffer(pH7.0) = 60/40 | Acetonitrile/ 10mmol/l CH3COONH4 = 60/40 |
 |
 |
Condition |
Column Size |
4.6 mm I.D. x 75 mm |
Flow Rate |
1.0 ml/min |
Temperature |
40°C |
Detection |
UV 254 nm |
Sample |
1. Amitriptyline 2. Propylbenzene (I.S.) |
0.2mg/ml 2.0mg/ml |
 |
|
Inj. Vol |
1.0 µl |
Analysis of standards in USP Methods
COSMOSIL C18-EB |
 |
Conditions |
Column Size |
4.6 mm I.D. x 100 mm |
Mobile Phase |
A; Acetonitrile/ 36.7mmol/l Phosphate Buffer(pH7.6) = 10/90 B; Acetonitrile/ 36.7mmol/l Phosphate Buffer(pH7.6) = 80/20 B conc. 0→ 20%(0→ 10min), 20→ 100%(10→ 30min) |
Flow Rate |
1.0 ml/min |
Temperature |
40°C |
Detection |
UV302 nm |
Sample |
1. Omeprazole related compound A
2. Omeprazole
|
0.001mg/ml
0.001mg/ml
|
|
Inj. Vol: |
20 µl |
USP 37(3) Esomeprazole Magnesium Delayed-Release Capsules, Impurities |
Ordering Information
Particle Size |
Column Dimension |
Part Number |
3um |
2.0 mm I.D. x 50 mm |
09794-21 |
3um |
2.0 mm I.D. x 75 mm |
09795-11 |
3um |
2.0 mm I.D. x 100 mm |
09796-01 |
3um |
2.0 mm I.D. x 150 mm |
09797-91 |
3um |
2.0 mm I.D. x 250 mm |
09798-81 |
3um |
3.0 mm I.D. x 50 mm |
09799-71 |
3um |
3.0 mm I.D. x 75 mm |
09800-21 |
3um |
3.0 mm I.D. x 100 mm |
09811-81 |
3um |
3.0 mm I.D. x 150 mm |
09814-51 |
3um |
3.0 mm I.D. x 250 mm |
09827-91 |
3um |
4.6 mm I.D. x 50 mm |
09840-01 |
3um |
4.6 mm I.D. x 75 mm |
09841-91 |
3um |
4.6 mm I.D. x 100 mm |
09842-81 |
3um |
4.6 mm I.D. x 150 mm |
09843-71 |
3um |
4.6 mm I.D. x 250 mm |
09844-61 |
Guard Columns |
- |
4.6mm Guard Cartridge Holder
|
38009-79
|
3um |
4.6 mm I.D. x 10 mm |
09839-41 |