The hydrophilic interaction chromatography is a variation of normal phase chromatography where a polar stationary phase is used with a mobile phase which contains a high concentration of water miscible organic solvent and a low concentration of aqueous eluent. The main retention mechanism is the partitioning of the polar analytes between the polar stationary and the non-polar mobile phase. As it is also called “aqueous normal phase”, the elution order is similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity
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