Cosmosil COSMOSIL Cholester COSMOSIL Cholester HPLC Columns.. Product #: VEL00489

COSMOSIL Cholester

COSMOSIL Cholester

Brand: Cosmosil
Product Code: VEL00489

COSMOSIL Cholester HPLC Columns

COSMOSIL Cholester StructureCOSMOSIL Cholester is a reversed phase HPLC column with cholesteryl groups bonded silica packing material, which provides equivalent hydrophobicity like traditional alkyl group bonded silica packing materials (C18, C30). However, Cholester offers strong molecular shape selectivity for hydrophobic compounds to yield unique and reproducible separation patterns following the same analytical conditions used with other ODS columns.

 Main Characteristics

  • Strong Stereoselectivity for hydrophobic compounds

 

COSMOSIL Cholester
 Base Material Silica Gel
 Particle Sizes Available 2.5, 5um
 Pore Size 120Å
 Surface Area 300m2/g
 pH Range 2.0 - 8.0
 USP Code -
 Carbon Load 20%

 

Hydrophobic Interaction

Figure 1 shows the comparison of hydrophobic interactions with competitive C18 columns. Cholester provides the same hydrophobicity as alkyl group bonded types (C18, C30). It is not necessary to change the analytical conditions when replacing C18 or C30 columns with Cholester.

 COSMOSIL Cholester 1

Figure1. Comparison of hydrophobic interactions

Comparison of Molecular Shape Selectivity

The stationary phase of Cholester has very rigid structures and can distinguish different molecular shapes. Cholester offers improved separation for structurally similar compounds that are difficult to analyze with alkyl group bonded materials (C18 and C30). As in the following example Cholester retains planar Triphenylene longer than stereoscopic o-Terphenyl.

 COSMOSIL Cholester 2

 COSMOSIL Cholester 3

COSMOSIL Cholester 4

Condition
Column Size 4.6 mm I.D. x 150 mm
Mobile phase Methanol : Water = 90 : 10
Flow rate 1.0 ml/min
Temperature 30 °C
Detection UV 254 nm
Sample 1. o-Terphenyl 0.10 µg                     2. Triphenylene 0.01 µg

 

Improvement in Separation

COSMOSIL Cholester provides enhanced selectivity over traditional C18 columns and offers greater performance in separating isomers or other closely related compounds. COSMOSIL Cholester is ideal for method development and serves as an excellent alternative to traditional C18 columns. The figure below shows analytical data of chloroacetophenone isomers. These isomers are difficult to separate with C18 and C30, but they are well resolved by COSMOSIL Cholester.

 COSMOSIL Cholester 5

Efficiency of Preparative Separation

 

 

 

The figure below shows the comparison of efficiency of preparative separation with a C18 column. Both columns show good separation. However, sample loading capacity for preparative separations can be affected by a slight difference in separation ability. COSMOSIL Cholester can load 4 times of sample volume compared with C18 columns.

 

COSMOSIL Cholester 6

 

Condition
Column Size 4.6 mm I.D. x 150 mm Detection UV 254 nm
Mobile phase Methanol : Water = 100 : 0 Sample
  1. Bibenzyl
  2. Anthracene
Flow rate 1.0ml/min
Temperature 30°C

 

Applications

Catechin
COSMOSIL Cholester 7 COSMOSIL Cholester Arrow COSMOSIL Cholester 8

 

Condition
Column Size 4.6 mm I.D. x 150 mm Sample
  1. Caffeine 0.12 µg
  2. Epigallocatechin (EGC) 1.20 µg
  3. Catechin 0.60 µg
  4. Epicatechin 0.60 µg
  5. Epigallocatechin Gallate 0.24 µg
  6. Epicatechin Gallate (ECG) 0.30 µg
Mobile Phase A : Acetonitrile : 20 mmol/l Phosphate Buffer (pH2.5) = 10 : 90       B : Acetonitrile : 20 mmol/l Phosphate Buffer (pH2.5) = 30 : 70       B : conc. 0 → 100% 20 min Linear Gradient
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 280 nm

 

Saikosaponin

COSMOSIL Cholester 9

Condition
Column Size 4. 6mm I.D. x 150 mm Sample
  1. Saikosaponin c 1.5 µg
  2. Saikosaponin a 1.5 µg
  3. Saikosaponin b2 1.5 µg
  4. Saikosaponin b1 1.5 µg
  5. Saikosaponin d 1.5 µg

 

Ordering Information

Particle Size Description Column Dimensions Part Number
5um COSMOSIL Cholester Packed Column 1.0mmI.D. x 150mm 05968-71
5um COSMOSIL Cholester Packed Column 1.0mmI.D. x 250mm 05969-61
5um COSMOSIL Cholester Packed Column 2.0mmI.D. x 30mm 08565-51
5um COSMOSIL Cholester Packed Column 2.0mmI.D. x 50mm 06352-91
5um COSMOSIL Cholester Packed Column 2.0mmI.D. x 100mm 06948-01
5um COSMOSIL Cholester Packed Column 2.0mmI.D. x 150mm 05971-11
5um COSMOSIL Cholester Packed Column 2.0mmI.D. x 250mm 05972-01
5um COSMOSIL Cholester Packed Column 3.0mmI.D. x 150mm 05973-91
5um COSMOSIL Cholester Packed Column 3.0mmI.D. x 250mm 05974-81
5um COSMOSIL Cholester Packed Column 4.6mmI.D. x 50mm 06359-21
5um COSMOSIL Cholester Packed Column 4.6mmI.D. x 100mm 06591-61
5um COSMOSIL Cholester Packed Column 4.6mmI.D. x 150mm 05976-61
5um Cholester Packed Column 3Lot Set 4.6mmI.D. x 150mm 07970-03
5um COSMOSIL Cholester Packed Column 4.6mmI.D. x 250mm 05977-51
5um COSMOSIL Cholester Packed Column 10.0mmI.D. x 150mm 08011-91
5um COSMOSIL Cholester Packed Column 10.0mmI.D. x 250mm 05979-31
5um COSMOSIL Cholester Packed Column 20.0mmI.D. x 150mm 06088-71
5um COSMOSIL Cholester Packed Column 20.0mmI.D. x 250mm 05982-71
5um COSMOSIL Cholester Packed Column 28.0mmI.D. x 250mm 05985-41
2.5um COSMOSIL 2.5Cholester Packed Column 2.0mmI.D. x 50mm 09000-01
2.5um COSMOSIL 2.5Cholester Packed Column 2.0mmI.D. x 70mm 09047-11
2.5um COSMOSIL 2.5Cholester Packed Column 2.0mmI.D. x 100mm 09048-01
2.5um