COSMOSIL Cholester HPLC Columns
COSMOSIL Cholester is a reversed phase HPLC column with cholesteryl groups bonded silica packing material, which provides equivalent hydrophobicity like traditional alkyl group bonded silica packing materials (C18, C30). However, Cholester offers strong molecular shape selectivity for hydrophobic compounds to yield unique and reproducible separation patterns following the same analytical conditions used with other ODS columns.
Main Characteristics
COSMOSIL Cholester | |
Base Material | Silica Gel |
Particle Sizes Available | 2.5, 5um |
Pore Size | 120Å |
Surface Area | 300m2/g |
pH Range | 2.0 - 8.0 |
USP Code | - |
Carbon Load | 20% |
Figure 1 shows the comparison of hydrophobic interactions with competitive C18 columns. Cholester provides the same hydrophobicity as alkyl group bonded types (C18, C30). It is not necessary to change the analytical conditions when replacing C18 or C30 columns with Cholester.
Figure1. Comparison of hydrophobic interactions
The stationary phase of Cholester has very rigid structures and can distinguish different molecular shapes. Cholester offers improved separation for structurally similar compounds that are difficult to analyze with alkyl group bonded materials (C18 and C30). As in the following example Cholester retains planar Triphenylene longer than stereoscopic o-Terphenyl.
Condition | |
---|---|
Column Size | 4.6 mm I.D. x 150 mm |
Mobile phase | Methanol : Water = 90 : 10 |
Flow rate | 1.0 ml/min |
Temperature | 30 °C |
Detection | UV 254 nm |
Sample | 1. o-Terphenyl 0.10 µg 2. Triphenylene 0.01 µg |
COSMOSIL Cholester provides enhanced selectivity over traditional C18 columns and offers greater performance in separating isomers or other closely related compounds. COSMOSIL Cholester is ideal for method development and serves as an excellent alternative to traditional C18 columns. The figure below shows analytical data of chloroacetophenone isomers. These isomers are difficult to separate with C18 and C30, but they are well resolved by COSMOSIL Cholester.
The figure below shows the comparison of efficiency of preparative separation with a C18 column. Both columns show good separation. However, sample loading capacity for preparative separations can be affected by a slight difference in separation ability. COSMOSIL Cholester can load 4 times of sample volume compared with C18 columns.
Condition | |||
---|---|---|---|
Column Size | 4.6 mm I.D. x 150 mm | Detection | UV 254 nm |
Mobile phase | Methanol : Water = 100 : 0 | Sample |
|
Flow rate | 1.0ml/min | ||
Temperature | 30°C |
Condition | |||
---|---|---|---|
Column Size | 4.6 mm I.D. x 150 mm | Sample |
|
Mobile Phase | A : Acetonitrile : 20 mmol/l Phosphate Buffer (pH2.5) = 10 : 90 B : Acetonitrile : 20 mmol/l Phosphate Buffer (pH2.5) = 30 : 70 B : conc. 0 → 100% 20 min Linear Gradient | ||
Flow Rate | 1.0 ml/min | ||
Temperature | 30°C | ||
Detection | UV 280 nm |
Condition | |||
---|---|---|---|
Column Size | 4. 6mm I.D. x 150 mm | Sample |
|
Ordering Information
Particle Size | Description | Column Dimensions | Part Number |
5um | COSMOSIL Cholester Packed Column | 1.0mmI.D. x 150mm | 05968-71 |
5um | COSMOSIL Cholester Packed Column | 1.0mmI.D. x 250mm | 05969-61 |
5um | COSMOSIL Cholester Packed Column | 2.0mmI.D. x 30mm | 08565-51 |
5um | COSMOSIL Cholester Packed Column | 2.0mmI.D. x 50mm | 06352-91 |
5um | COSMOSIL Cholester Packed Column | 2.0mmI.D. x 100mm | 06948-01 |
5um | COSMOSIL Cholester Packed Column | 2.0mmI.D. x 150mm | 05971-11 |
5um | COSMOSIL Cholester Packed Column | 2.0mmI.D. x 250mm | 05972-01 |
5um | COSMOSIL Cholester Packed Column | 3.0mmI.D. x 150mm | 05973-91 |
5um | COSMOSIL Cholester Packed Column | 3.0mmI.D. x 250mm | 05974-81 |
5um | COSMOSIL Cholester Packed Column | 4.6mmI.D. x 50mm | 06359-21 |
5um | COSMOSIL Cholester Packed Column | 4.6mmI.D. x 100mm | 06591-61 |
5um | COSMOSIL Cholester Packed Column | 4.6mmI.D. x 150mm | 05976-61 |
5um | Cholester Packed Column 3Lot Set | 4.6mmI.D. x 150mm | 07970-03 |
5um | COSMOSIL Cholester Packed Column | 4.6mmI.D. x 250mm | 05977-51 |
5um | COSMOSIL Cholester Packed Column | 10.0mmI.D. x 150mm | 08011-91 |
5um | COSMOSIL Cholester Packed Column | 10.0mmI.D. x 250mm | 05979-31 |
5um | COSMOSIL Cholester Packed Column | 20.0mmI.D. x 150mm | 06088-71 |
5um | COSMOSIL Cholester Packed Column | 20.0mmI.D. x 250mm | 05982-71 |
5um | COSMOSIL Cholester Packed Column | 28.0mmI.D. x 250mm | 05985-41 |
2.5um | COSMOSIL 2.5Cholester Packed Column | 2.0mmI.D. x 50mm | 09000-01 |
2.5um | COSMOSIL 2.5Cholester Packed Column | 2.0mmI.D. x 70mm | 09047-11 |
2.5um | COSMOSIL 2.5Cholester Packed Column | 2.0mmI.D. x 100mm | 09048-01 |
2.5um |