Cosmosil COSMOSIL C18-PAQ-II COSMOSIL C18-PAQ-II HPLC Columns.. Product #: VEL00488

COSMOSIL C18-PAQ-II

COSMOSIL C18-PAQ-II

Brand: Cosmosil
Product Code: VEL00488

COSMOSIL C18-PAQ HPLC Columns

COSMOSIL C18-PAQ structureThe COSMOSIL C18-PAQ is a new member of our C18 column family which already includes the monomeric type MS-II and the polymeric type AR-II. This new column maintains stable retention time even in 100% aqueous mobile phases. The new polymeric linking style gives this column a strong acidic resistance so that it is compatible with mobile phases of acidic pH that can permanently damage conventional octadecyl stationary phases. This type is especially good for separation of hydrophilic compounds.

 Main Characteristics

  • Stable Performance under 100% Aqueous Conditions
  • Column of choice for Hydrophilic Compounds

 

COSMOSIL C18-PAQ
 Base Material Silica Gel
 Particle Sizes Available 5, 15um
 Pore Size 120Å
 Surface Area 330m2/g
 pH Range 2.0 - 7.5
 USP Code L1
 Carbon Load 11%

 

Stable Performance under 100% Aqueous Conditions

The figure shows the change of retention time for thymine with 100% aqueous mobile phase (20 mmol/l phosphate buffer pH7). The sample was analyzed 4 times. Flow of mobile phase was then stopped for 1 hour. The sample was analyzed under the same condition again after 1 hour. The conventional C18 column showed change of retention time, but COSMOSIL C18-PAQ maintained stable retention time.

 COSMOSIL C18-PAQ 1

Applications

Organic Acid

 COSMOSIL C18-PAQ 2

Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase 20 mmol/l Phosphoric Acid
Flow rate 1.0ml/min
Temperature 30°C
Detection UV 210 nm 0.16AUFS
Sample
  1. Glycolic Acid 6.3 µg
  2. Malic Acid 6.3 µ g
  3. Lactic Acid 13 µg
  4. Citric Acid 6.3 µ g
  5. Maleic Acid 0.063 µg
  6. Fumaric Acid 0.031 µg

 

 
COSMOSIL C18-PAQ 3
Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase 20 mmol/l Phosphoric Acid
Flow Rate 1.0ml/min
Temperature 30°C
Detection UV 210 nm 0.16AUFS
Sample
  1. Tartaric Acid 4.0 µg
  2. Acetic Acid 13 µg
  3. Succinic Acid 13 µg
  4. Propionic Acid 13 µg
 
Nucleic-acid Base

 COSMOSIL C18-PAQ 4

Condition
Column Size 4.6 mm I.D. x 150 mm
Mobile Phase 20 mmol/l Phosphate Buffer (pH7)
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 254 nm 0.16AUFS
Sample
  1. Cytosine 0.1 µg
  2. Uracil 0.1 µg
  3. Guanine 0.1 µg
  4. Thymine 0.2 µg
  5. Adenine 0.05 µg
 
dNTP

 COSMOSIL C18-PAQ 5

Condition
Column Size 4.6 mm I.D. x 250 mm
Mobile Phase 20 mmol/l Phosphate Buffer (pH7)
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 260 nm 0.16 AUFS
Sample
  1. dCTP
  2. dTTP
  3. dGTP
  4. dATP 1 mmol/ each 0.5 µl
 
Ascorbic Acid

 COSMOSIL C18-PAQ 6

Condition
Column Size 4.6 mm I.D. x 250 mm mm
Mobile Phase 20 mmol/l Phosphoric Acid
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 245 nm 0.16 AUFS
Sample Ascorbic Acid (Vitamin C) 0.1 µg
 
2-Phenylethyl Glycoside
COSMOSIL C18-PAQ 7

COSMOSIL C18-PAQ 8

 

Condition
Column Size 4.6 mm I.D. x 150 mm
Mobile Phase Acetonitrile : Methanol : Water = 8 : 4 : 88
Flow Rate 1.0 ml/min
Temperature 30°C
Detection UV 210 nm 0.064 AUFS
Sample 10.5 MPa

Ordering Information 

 

Particle Size Description Column Dimensions Part Number
5um COSMOSIL C18-PAQ   Column 1.0 x 50mm 05792-61
5um COSMOSIL C18-PAQ Column 1.0 x 150mm 05793-51
5um COSMOSIL C18-PAQ Column 2.0 x 30mm 05878-51
5um COSMOSIL C18-PAQ Column 2.0 x 50mm 05794-41
5um COSMOSIL C18-PAQ Column 2.0 x 100mm 05470-71
5um COSMOSIL C18-PAQ Column 2.0 x 150mm 34449-71
5um COSMOSIL C18-PAQ Column 2.0 x 250mm 05795-31
5um COSMOSIL C18-PAQ Column 3.0 x 100mm 05796-21
5um COSMOSIL C18-PAQ Column 3.0 x 150mm 05797-11
5um COSMOSIL C18-PAQ Column 3.0 x 250mm 05798-01
5um COSMOSIL C18-PAQ Column 4.6 x 30mm 05879-41
5um COSMOSIL C18-PAQ Column 4.6 x 50mm 34451-21
5um COSMOSIL C18-PAQ Column 4.6 x 100mm 05799-91
5um COSMOSIL C18-PAQ Column 4.6 x 150mm 02486-71
5um COSMOSIL C18-PAQ Column 4.6 x 250mm 02485-81
5um COSMOSIL C18-PAQ Column 6.0 x 150mm 34419-61
5um COSMOSIL C18-PAQ Column 6.0 x 250mm 05800-41
5um COSMOSIL C18-PAQ Column 10.0 x 50mm 05801-31
5um COSMOSIL C18-PAQ Column 10.0 x 150mm 34466-41
5um COSMOSIL C18-PAQ Column 10.0 x 250mm 34376-21
5um COSMOSIL C18-PAQ Column 20.0 x 150mm 34476-11
5um COSMOSIL C18-PAQ Column 20.0 x 250mm 34373-51
5um COSMOSIL C18-PAQ Column 28.0 x 250mm