Cosmosil COSMOSIL C18-EB COSMOSIL C18-EB HPLC Columns.. Product #: VEL00485

COSMOSIL C18-EB

COSMOSIL C18-EB

Brand: Cosmosil
Product Code: VEL00485

COSMOSIL C18-EB HPLC Columns

Conventionally end-capped C18 columns still have many residual silanol groups on the silica surface of stationary phase that can form ionic bonds with basic compounds. The resulting peak tailing makes accurate quantification of analytes difficult, especially in trace analyses. COSMOSIL 3C18-EB with a better end-capping treatment offers improved peak shape and separation particularly for basic compounds.

 Main Characteristics

  • Improved separation for acidic or metal coordination compounds
  • Simple mobile phase conditions
  • Excellent for Basic Compounds 
COSMOSIL C18-EB
 Base Material Silica Gel
 Particle Sizes Available 3um
 Pore Size 120A 
 Surface Area  300m2/g
 pH Range 2-10 
 USP Code  L1
 Carbon Load 14.5% 

 

Comparison  (Basic compounds)
COSMOSIL C18-EBCompetitor A C18, 3 µmCompetitor B C18, 3 µmCompetitor C C18, 3 µm
COSMOSIL C18-EB BC1 COSMOSIL C18-EB BC2 COSMOSIL C18-EB BC3 COSMOSIL C18-EB BC4
 
Conditions
Column Size 4.6 mm I.D. x 75 mm
Mobile Phase Acetonitrile/ 20mmol/l Phosphate       Buffer(pH7.0) = 60/40
Flow Rate 1.0 ml/min
Temperature 40°C
Detection UV 254 nm
Sample
1.  Amitriptyline              2. Propylbenzene (I.S.) 0.2mg/ml             2.0mg/ml COSMOSIL C18-EB BC5
Inj. Vol 2.0 µl

 

Analysis of Acidic or Metal Coordination Compounds

Acidic or metal coordination compounds can also form bonds with residual silanol groups and metal impurities causing peak tailing. COSMOSIL 3C18-EB with a better end-capping treatment and high purity silica gel offers improved peak shape and separation for acidic or metal coordination compounds.

Acidic Compounds
COSMOSIL C18-EB
COSMOSIL C18-EB AC1
 
Conditions
Column Size 4.6 mm I.D. x 75 mm
Mobile Phase Acetonitrile/ 20mmol/l Phosphate       Buffer(pH2.5) = 30/70
Flow Rate 1.0 ml/min
Temperature 40°C
Detection UV254 nm
Sample
1. Salicylic Acid             2. Ethyl p-Hydroxybenzoate (I.S.) 1.0 mg/ml             0.05 mg/ml COSMOSIL C18-EB AC2
 
Metal Coordination Compounds
COSMOSIL C18-EB
COSMOSIL C18-EB MC1
 
Conditions
Column Size 4.6 mm I.D. x 75 mm
Mobile Phase Acetonitrile/ 20mmol/l       Phosphoric Acid = 10/90
Flow Rate 1.0 ml/min
Temperature 40°C
Detection UV 254 nm
Sample
1. Oxine-copper             2. Caffeine (I.S.) 0.03 mg/ml             0.3 mg/ml COSMOSIL C18-EB MC2
Inj. Vol 2.0 µl

 

Analysis under Ammonium Acetate Buffer

COSMOSIL C18-EB produces sharp peaks using ammonium acetate buffer in LC-MS.

Acetonitrile/ 20mmol/l Phosphate buffer(pH7.0) = 60/40Acetonitrile/ 10mmol/l CH3COONH4 = 60/40
COSMOSIL C18-EB AAB1 COSMOSIL C18-EB AAB2

Condition
Column Size 4.6 mm I.D. x 75 mm
Flow Rate 1.0 ml/min
Temperature 40°C
Detection UV 254 nm
Sample
1.  Amitriptyline              2. Propylbenzene (I.S.) 0.2mg/ml             2.0mg/ml COSMOSIL C18-EB AAB3
Inj. Vol 1.0 µl

 

Analysis of standards in USP Methods

COSMOSIL C18-EB
COSMOSIL C18-EB USP1
 
Conditions
Column Size 4.6 mm I.D. x 100 mm
Mobile Phase A; Acetonitrile/ 36.7mmol/l Phosphate Buffer(pH7.6) = 10/90       B; Acetonitrile/ 36.7mmol/l Phosphate Buffer(pH7.6) = 80/20 B conc. 0→ 20%(0→ 10min), 20→ 100%(10→ 30min)
Flow Rate 1.0 ml/min
Temperature 40°C
Detection UV302 nm
Sample

1. Omeprazole related compound A            

2. Omeprazole

0.001mg/ml            

0.001mg/ml

Inj. Vol: 20 µl
USP 37(3) Esomeprazole Magnesium Delayed-Release Capsules, Impurities

 

Ordering Information

Particle Size Column Dimension Part Number
3um 2.0 mm I.D. x 50 mm 09794-21
3um 2.0 mm I.D. x 75 mm 09795-11
3um 2.0 mm I.D. x 100 mm 09796-01
3um 2.0 mm I.D. x 150 mm 09797-91
3um 2.0 mm I.D. x 250 mm 09798-81
3um 3.0 mm I.D. x 50 mm 09799-71
3um 3.0 mm I.D. x 75 mm 09800-21
3um 3.0 mm I.D. x 100 mm 09811-81
3um 3.0 mm I.D. x 150 mm 09814-51
3um 3.0 mm I.D. x 250 mm 09827-91
3um 4.6 mm I.D. x 50 mm 09840-01
3um 4.6 mm I.D. x 75 mm 09841-91
3um 4.6 mm I.D. x 100 mm 09842-81
3um 4.6 mm I.D. x 150 mm 09843-71
3um 4.6 mm I.D. x 250 mm 09844-61
Guard Columns
-
4.6mm Guard Cartridge Holder
38009-79
3um 4.6 mm I.D. x 10 mm 09839-41