Pipette Tip SPE Cartridges
Pipette Tip SPE Cartridges
SiliaPrep Tips for Micro Sample Preparation
SiliaPrep Tips Micro-SPE Cartridges are designed for micro-purification and micro-extraction of femtomole (fmol) to picomole (pmol) quantities of analytes prior to the analysis by chromatographic techniques and / or mass spectrometry.
The constant improvement in these techniques of analysis has allowed scientists to decrease the limit of quantification in several applications. This lower limit has pushed SPE manufacturers to design new SPE cartridges accepting smaller volumes of analyte.
These tips are specially designed to achieve extraction and purification of small molecules, peptides, phosphopeptides and proteins.
They are packed with our SiliaBond functionalized silica gels and specialty phases to cover the broadest spectrum of applications requiring small volume of analytes.
The phases are embedded directly in the inner surface of the tip to provide consistent flow rates. Finally, no glue is used during packing procedures in order to prevent any contamination of the analyte.
Using SiliaPrep Tips and Tips Micro-SPE Cartridges guarantees the following benefits:
- SiliaPrep Tips Micro-SPE Cartridges
- Simple, fast analyte retention & elution with minimal loss
- Sorbents directly embedded into inner cartridge wall
- High binding capacity
- No back-pressure
SiliaPrep Micro-SPE Cartridges Sizes
SiliaPrep Tips Micro-SPE Cartridges are available in 3 different cartridge formats, based on the binding capacity of each embedded sorbent.
| Tip Volume (μL) | Sample Volume (μL) | Binding Capacity (μg) | Sorbent Weight (μg) | Product Number |
|---|---|---|---|---|
| 1 - 10 | 0.5 - 10 | 1 | 30 | -T1 |
| 10 - 200 | 2 - 25 | 2.5 | 75 | -T2 |
| 10 - 200 | 5 - 50 | 15 | 400 | -T3 |
SiliaPrep XL Tips for Bigger Volumes
For bigger volumes, we also offer SiliaPrep XL Tips Micro-SPE Cartridges, in 3 different formats. Please note these tips are top loading instead of by aspiration.
| Tip Volume (μL) | Sample Volume (μL) | Binding Capacity (μg) | Sorbent Weight (μg) | Product Number |
|---|---|---|---|---|
| 1 - 10 | 1 - 10 | 400 | 4 | -T1 |
| 10 - 200 | 2 - 25 | 1,000 | 10 | -T2 |
| 100 - 1,000 | 20 - 1,000 | 5,000 | 50 | -T3 |
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SiliaPrep Tips Sorbent Descriptions and Ordering Information |
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SiliaPrep Tips Sorbent |
Description |
Product Number |
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10 µL / 30 µg |
200 µL / 75 µg |
200 µL / 400 µg |
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C18 |
Highest hydrophobic character sorbent. Mainly used for small peptides and small molecules purification & enrichment or protein / peptide desalting analysis. |
SPET-C18-T1 |
SPET-C18-T2 |
SPET-C18-T3 |
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C8 |
Mid-level hydrophobic sorbent. Mainly used for sample treatment of proteins and peptides requiring a lower hydrophobic capacity, and protein / peptide desalting analysis. |
SPET-C8-T1 |
SPET-C8-T2 |
SPET-C8-T3 |
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C4 |
Lowest hydrophobic character sorbent. Mainly used for protein purification & enrichment or protein / peptide desalting analysis. |
SPET-C4-T1 |
SPET-C4-T2 |
SPET-C4-T3 |
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HLB |
Polymeric sorbent with an hydrophilic-lipophilic balance. Mainly used for hydrophobic and neutral molecules enrichment. |
SPET-HLB-T1 |
SPET-HLB-T2 |
SPET-HLB-T3 |
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DVB |
Higly hydrophobic polymeric sorbent. Mainly used for hydrophobic and neutral molecules enrichment. |
SPET-DVB-T1 |
SPET-DVB-T2 |
SPET-DVB-T3 |
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Carbon Black |
Hydrophilic and hydrophobic character. Mainly used for purification of oligosaccharides and other macromolecules containing sugar functions, or protein / peptide desalting. |
SPET-CB-T1 |
SPET-CB-T2 |
SPET-CB-T3 |
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HILIC |
Moderatly polar sorbent. Mainly used for proteins removal, peptides desalting, small molecules enrichment and detergent removal (broad spectrum of detergents). |
SPET-HI-T1 |
SPET-HI-T2 |
SPET-HI-T3 |
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Cyano (CN) |
Both polar and hydrophobic character. Mainly used for hydrophilic and neutral molecules enrichment. |
SPET-CN-T1 |
SPET-CN-T2 |
SPET-CN-T3 |
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Silica |
Most polar sorbent. Mainly used for hydrophilic molecules enrichment. |
SPET-SI-T1 |
SPET-SI-T2 |
SPET-SI-T3 |
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SAX |
Strong anion exchanger sorbent. Mainly used for weak acids enrichment. |
SPET-SAX-T1 |
SPET-SAX-T2 |
SPET-SAX-T3 |
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NH (WAX) |
Weak anion exchanger sorbent. Mainly used for strong acids enrichment (phosphopeptides and phosphoproteins). |
SPET-NH2-T1 |
SPET-NH2-T2 |
SPET-NH2-T3 |
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SCX |
Strong cation exchanger sorbent. Mainly used for weak bases enrichment. |
SPET-SCX-T1 |
SPET-SCX-T2 |
SPET-SCX-T3 |
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WCX |
Weak cation exchanger sorbent. Mainly used for strong bases enrichment. |
SPET-WCX-T1 |
SPET-WCX-T2 |
SPET-WCX-T3 |
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SAX Polymeric |
Polymeric sorbent functionalized by a strong anion exchanger. Mainly used for weak acids enrichment. |
SPET-XSAX-T1 |
SPET-XSAX-T2 |
SPET-XSAX-T3 |
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WAX Polymeric |
Polymeric sorbent functionalized by a weak anion exchanger. Mainly used for strong acids enrichment (phosphopeptides and phosphoproteins). |
SPET-XWAX-T1 |
SPET-XWAX-T2 |
SPET-XWAX-T3 |
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SCX Polymeric |
Polymeric sorbent functionalized by a strong cation exchanger. Mainly used for weak bases enrichment. |
SPET-XSCX-T1 |
SPET-XSCX-T2 |
SPET-XSCX-T3 |
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WCX Polymeric |
Polymeric sorbent functionalized by a weak cation exchanger. Mainly used for strong bases enrichment. |
SPET-XWCX-T1 |
SPET-XWCX-T2 |
SPET-XWCX-T3 |
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TiO |
High selectivity for multiple phosphorylated peptides. Mainly used for phosphopeptide enrichment and phospholipid removal. |
SPET-TI-T1 |
SPET-TI-T2 |
SPET-TI-T3 |
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ZrO |
High selectivity for mono-phosphorylated peptides. Mainly used for phosphopeptide enrichment and phospholipid removal. |
SPET-ZR-T1 |
SPET-ZR-T2 |
SPET-ZR-T3 |
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TiO / ZrO 2 2 |
Excellent alternative for the enrichment of a broad spectrum of phosphopeptides (litterature suggests only 30 % overlap in phosphopeptides isolated by TiO versus ZrO ) and phospholipid removal. 2 2 |
SPET-TIZR-T1 |
SPET-TIZR-T2 |
SPET-TIZR-T3 |
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SDS Removal |
Used to remove SDS from peptides and proteins. |
SPET-SDS-T1 |
SPET-SDS-T2 |
SPET-SDS-T3 |
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Trypsin |
Used to cleave proteins and peptides at the C-terminal side, with minimal protease contaminants. |
SPET-TRYP-T1 |
SPET-TRYP-T2 |
SPET-TRYP-T3 |
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Fluoro |
Fluorinated sorbent. Mainly used for fluorine containing molecules enrichment. |
SPET-FL-T1 |
SPET-FL-T2 |
SPET-FL-T3 |
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Metal Scavengers |
Mainly used to lower the residual metal concentration of various metal complexes (Pd, Pt, Rh, Ru, Ni, Sn, etc). Choice of 9 metal scavenging sorbents: Cysteine, DMT, Imidazole, Diamine, TAAcOH, TAAcONa, Thiol, Thiourea and Triamine. |
SPET-CYS-T1 SPET-DMT-T1 SPET-IMIDAZ-T1 SPET-PSA-T1 SPET-TAACOH-T1 SPET-TAACONA-T1 SPET-THIOL-T1 SPET-THIOUREA-T1 SPET-TRINH2-T1 |
SPET-CYS-T2 SPET-DMT-T2 SPET-IMIDAZ-T2 SPET-PSA-T2 SPET-TAACOH-T2 SPET-TAACONA-T2 SPET-THIOL-T2 SPET-THIOUREA-T2 SPET-TRINH2-T2 |
SPET-CYS-T3 SPET-DMT-T3 SPET-IMIDAZ-T3 SPET-PSA-T3 SPET-TAACOH-T3 SPET-TAACONA-T3 SPET-THIOL-T3 SPET-THIOUREA-T3 SPET-TRINH2-T3 |
Note: Add "XL" after "SPET" for ordering SiliaPrep XL Tips. For example SPETXL-C18-T1.
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Product Selection Guide by Manufacturer |
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SiliCycle |
Waters |
Phenomenex |
Agilent |
Biotage |
Macherey-Nagel |
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SiliaPrep C18 Plus |
Sep-Pak® tC18 |
Strata® C18-E |
Bond Elut® C18 |
Isolute® C18 (EC) |
Chromabond® C18 ec |
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SiliaPrep C18 nec |
Strata® C18-U |
Bond Elut® C18 OH |
Isolute® C18 |
Chromabond® C18 |
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SiliaPrep C18 WPD |
Sep-Pak® C18 |
Strata® C18-T |
Bond Elut® C18 EWP |
Isolute® MFC18 |
Chromabond® C18 ec f |
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SiliaPrep C8 |
Sep-Pak® C8 |
Strata® C8 |
Bond Elut® C8 |
Isolute® C8 (EC) |
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SiliaPrep C8 nec |
Isolute® C8 |
Chromabond® C8 |
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SiliaPrep Phenyl (PH) |
Strata® Phenyl |
Bond Elut® PH |
Isolute® PH |
Chromabond® C H 6 5 |
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SiliaPrep PFP |
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SiliaPrep Cyano (CN) |
Sep-Pak® Cyanopropyl |
Strata® CN |
Bond Elut® Cyano (CN) |
Isolute® CN |
Chromabond® CN |
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SiliaPrep Diol nec |
Sep-Pak® Diol |
Bond Elut® Diol (2OH) |
Isolute® DIOL |
Chromabond® OH (Diol) |
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SiliaPrep Silica |
Strata® Silica (Si-1) |
Bond Elut® SI |
Isolute® SI |
Chromabond® SiOH |
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SiliaPrep Silica WPD |
Sep-Pak® Silica |
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SiliaPrep Florisil LP & Florisil PR |
Sep-Pak® Florisil® |
Strata® FL-PR (Florisil®) |
Bond Elut® Florisil |
Isolute® FL |
Chromabond® Florisil® (A, N, B) |
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SiliaPrep Alumina (Acidic, Neutral, Basic) |
Sep-Pak® Alumina (A, N, B) |
Strata® Alumina-N (AL-N) |
Bond Elut® Alumina (-A, -N, -B) |
Isolute® ALUMINA (AL-A, AL-N & AL-B) |
Chromabond® Alox |
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SiliaPrep SAX nec (TMA Chloride) |
Sep-Pak® Accell™ Plus QMA |
Strata® SAX |
Bond Elut® SAX |
Isolute® SAX |
Chromabond® SB |
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SiliaPrep SAX-2 nec (TMA Acetate) |
Isolute® PE-AX |
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SiliaPrep Carbonate |
Accell Plus QMA Carbonate Plus Light |
Isolute® Si-Carbonate (Si-TMA-CO3) |
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SiliaPrep Amine (WAX) |
Sep-Pak® Amino |
Strata® NH |
Bond Elut® NH2 |
Isolute® NH |
Chromabond® NH |
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SiliaPrep Tosic Acid (SCX) |
Strata® SCX |
Bond Elut® SCX |
Isolute® SCX-3 |
Chromabond® SA |
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SiliaPrep SCX-2 (Propylsulfonic Acid) |
Bond Elut® PRS |
Isolute® SCX-2 |
Chromabond® PSA |
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SiliaPrep WCX (Carboxylic Acid) |
Sep-Pak® Accell™ Plus CM |
Strata® WCX |
Bond Elut® CBA |
Isolute® CBA |
Chromabond® PCA |
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SiliaPrep PCB |
Bond Elut® PCB |
Chromabond® SA/SiOH |
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SiliaPrep CleanDRUG |
Strata® Screen-C |
Bond Elut® Certify |
Isolute® HCX |
Chromabond® Drug |
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SiliaPrep CleanENVI& SiliaPrep PAH |
Strata® PAH |
EnvirElut® |
Isolute® PAH |
Chromabond® C18 PAH |
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SiliaPrepX HLB |
Oasis® HLB |
Strata®-X |
Bond Elut® NEXUS |
Chromabond® HLB |
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SiliaPrepX DVB |
Bond Elut® ENV |
Isolute® 101 |
Chromabond® HR-X |
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SiliaPrepX SAX |
Oasis® MAX |
Strata®-X-A |
Bond Elut® Plexa PAX |
Evolute® Express AX |
Chromabond® HR-XA |
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SiliaPrepX WAX |
Oasis® WAX |
Strata®-X-AW |
Evolute® Express WAX |
Chromabond® HR-XAW |
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SiliaPrepX SCX |
Oasis® MCX |
Strata®-X-C |
Bond Elut® Plexa PCX |
Evolute® Express CX |
Chromabond® HR-XC |
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SiliaPrepX WCX |
Oasis® WCX |
Strata®-X-CW |
Bond Elut® NEXUS WCX |
Evolute® Express WCX |
Chromabond® HR-XCW |